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Box 8.5 Intrinsic bioremediation of the Exxon Valdez oil spill in Prince William Sound, Alaska The accident On March 24, 1989, at 12:03 a.m. the oil tanker Exxon Valdez hit Bligh Reef, Prince William Sound, Alaska. Eight of 11 cargo tanks ruptured, spilling 260,000 barrels (~40 million liters) of crude oil into Prince William Sound. There was immediate exposure of the intertidal and subtidal zones to oil. After 3 days of calm weather and little or no oil-spill-response effort, a major windstorm drove the oil slick southward towards Smith, Naked, and Knight Islands (Figure 1). The oil reached beaches in Chugach National Forest, Kenai Fjords National Park, and other parks and wildlife regions. The oil impacted sites reach as far as 1000 km away and ~2500 km of shoreline was contaminated. Immediate effects At the time of the accident, reproduction and migration was in progress for many sh, birds, and mammals. 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It is a high density 2D barcode symbology with fast readability. Findlay, R and FC Dobbs 1993 Quantitiative description of microbial communities using lipid analysis In: PF Kemp, BF Sherr, EB Sherr, and JJ Cole (eds) Handbook of Methods in Aquatic Microbial Ecology, pp 271 284 Lewis Publishers, Chelsea, MI Fisher, MM and EW Triplett 1999 Automated approach for ribosomal intergenic spacer analysis of microbial diversity and its application to freshwater bacterial communities Appl Environ Microbiol 65:4630 4636 Fliermans, CB and EL Schmidt 1975 Autoradiography and immuno uorescence combined for autoecological study of single cell activity with Nitrobacter as a model system Appl Microbiol 30: 676 684 Fred, EB, IL Baldwin, and E McCoy 1932 Root Nodule Bacteria and Leguminous Plants University of Wisconsin Studies in Science, No 5 University of Wisconsin, Madison, WI Frey, JC, ER Angert, and AN Pell. 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Requirements: Our ERP system uses integrated Crystal ... 2006 Assessment of biases associated with pro ling simple, model communities using terminal-restriction fragment length polymorphism-based analyses J Microbiol Methods 67:9 19 Ginige, MP, P Hugenholtz, H Daims, M Wagner, J Keller, and LL Blackall 2004 Use of stableisotope probing, full-cycle rRNA analysis, and uorescence in situ hybridization microautoradiography to study a methanol-fed denitrifying microbial community Appl Environ Microbiol 70: 588 596 Giovannoni, SJ, TB Britschgi, CL Moyer, and KG Field 1990 Genetic diversity on Sargasso Sea bacteriophytoplankton Nature 345:60 63 Giovannoni, SJ, L Bubbs, J-C Cho, et al 2005a Proteorhodopsin in the ubiquitous marine bacterium SARII Nature 438:82 85 Giovannoni, S J, L Bubbs, J-C Cho, et al 2005b Genomic streamlining in a cosmopolitan oceanic bacterium Science 309:1242 1245 Glud, RN, JK Gundersen, NP Revsbech, and BB Jorgensen 1994. 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