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Protocol 10.2. Differentiation of Human Chondroprogenitor Cells Reagents and Materials Sterile Differentiation medium (see Section 10.2.1.4) PBSA (see Section 10.2.2.4) Trypsin/EDTA: trypsin (0.05%) with EDTA (0.53 mM) in PBSA Centrifuge tubes, 15 mL Universal containers (30 mL) or centrifuge tubes (50 mL) with conical base Nonsterile Hemocytometer Microfuge Procedure (a) Aspirate growth medium from asks and discard. (b) Wash in PBSA and discard wash. (c) Add enough trypsin/EDTA to cover the base of the ask. (d) Incubate for 5 10 min at 37 C (check under microscope to see if cells are detached). (e) Resuspend cells, place in a 15-mL centrifuge tube, and centrifuge for 5 min at 300 g.

Figure 5.22 RTO behaviour in EGPRS (TimeStamp and SACK disabled)

The four modes are indicated by small, iconic buttons in the Tool area at the top left of a FileMaker window. Browse is the pencil, Find is the magnifying glass, Layout is the T-square, and Preview is the dog-eared piece of paper. The selected mode is highlighted, and its name appears in the text box above the buttons.

Summary

After the configuration constants comes the beginning of the definition of the main() function driving the program. Here, the database of feed entries included in the page from the previous program run is opened, with an empty list used by default if there s any problem in reading the file such as the file not yet existing on the first run of the program. Next, the aggregator databases are opened and the list of feeds is read in. The next part in Listing 17-2 continues the driver function with the start of feed processing.

< xml version= 1.0 encoding= utf-8 > <s:Application xmlns:fx= http://ns.adobe.com/mxml/2009 xmlns:s= library://ns.adobe.com/flex/spark xmlns:mx= library://ns.adobe.com/flex/mx > <s:states> <s:State name= State1 /> <s:State name= oneway /> </s:states> <fx:Style> @namespace mx library://ns.adobe.com/flex/mx ; @namespace s library://ns.adobe.com/flex/spark ; s|Label { font-weight:bold } </fx:Style> <mx:Image x= 23 y= 10 source= assets/airplane.png /> <s:Label text= Flight Search fontSize= 14 fontWeight= bold x= 104 y= 33 /> <s:Panel title= Select travel dates width= 421 height= 200 id= panel1 x= 23 y= 78 > <s:Label x= 10 y= 10 text= From (city or airport): /> <s:Label x= 214 y= 10 text= To (city or airport): /> <mx:DateField x= 61 y= 77 id= returnDate /> <s:TextInput x= 10 y= 36 id= departInput /> <s:Label x= 10 y= 79 text= Depart: /> <s:TextInput x= 214 y= 36 id= returnInput /> <s:Label x= 214 y= 79 text= Return: id= label1 includeIn= State1 /> <mx:DateField x= 270 y= 77 id= departDate includeIn= State1 /> <s:Group bottom= 0 width= 100% > <s:layout> <s:HorizontalLayout paddingLeft= 10 paddingRight= 10 paddingBottom= 10 /> </s:layout> continued

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