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A DNA-dependent RNA polymerase RT is used to make cDNA As a noun, sequence is the order of monomeric subunits in DNA or RNA or protein molecules For DNA, the sequence is the arrangement of A, T, G, and C bases it contains As a verb, to sequence is to determine the structure of a particular DNA, RNA, or protein molecule: for instance, the speci c sequence of nucleotides in a piece of DNA A way of determining the sequence of a large DNA fragment The large fragment is broken into many small pieces (~3 kb), and then each is taken up by a plasmid in an E coli host and sequenced By nding out where the 3 kb pieces overlap, the sequence of the larger DNA fragment becomes apparent.

s t a t e - n e x t <= t e s t - i n i t ; m e m <= 1 ; r w <= 0 ; a d d r <= 0 0 0 0 0 0 0 0 0 0 & s w ; d a t a - f 2 s <= ( o t h e r s = > 1 ) ; when w r - c l k l = > -- i n i d l e s t a t e o f s r a m - c t r l s t a t e - n e x t <= w r - c l k 2 ; mern <= 1 ; r w <= 0 ; a d d r <= c - s t d ; d a t a - f 2 s <= n o t c-std(DATA-W -1 downto 0 ) ; when w r - c l k 2 = > -- i n w r l s t a t e o f s r a r n - c t r l s t a t e - n e x t <= w r - c l k 3 ; when w r - c l k 3 = > -- i n wr2 s t a t e o f s r a r n - c t r l c - n e x t <= c - r e g + 1 ; i f c - n e x t =O t h e n s t a t e - n e x t <= t e s t - i n i t ;

Proposition 6.2:

Many regions of the original fragment will be sequenced several times; this overlap or clone coverage is necessary and allows assembly of the pieces.

(b )

Within the group of layout tools at the upper right of the Status Toolbar are two tools labeled Field and Part, respectively. When the mouse button is depressed with the cursor positioned over either of these tools, it takes the shape of a hand, indicating that you may drag from the tool to the layout area to add the corresponding element (field or part) to the current layout. When you release the mouse button over the layout, a field or part will be added at the location of the mouse coordinates.

where Fi,, and D are, respectively, the field tensor and covariant derivative for a color gauge field, and the coupling g entering the QCD terms through (10.33) is an effective coupling appropriate t o the interior of a hadron. The coefficient n is the dielectric constant introduced previously in the phenomenological discussion of confinement ($12.1). The field a = a ( & )is a classical scalar field; thus we consider only long wavelength effects and neglect loop diagrams for the u-field. Inside the hadron n = 1 and u(n) = 0, while outside in the normal vacuum K. = 0 and a(.) = avac 0. The energy # density is of the form

Able to store and process huge volumes of content in a timely fashion The service-oriented model offers a uniform means of describing and encapsulating activities at all layers in the Grid This model then needs to be underpinned by the appropriate processing and communication infrastructure to ensure it can deliver the desired performance Allow different stakeholders to retain ownership of their own content and processing capabilities, but to allow others access under the appropriate terms and conditions Each service owner retains control over the services that they make available to others They determine how the service is realized and set the policy for accessing the service Allow users to discover, transparently access and process relevant content wherever it may be located in the Grid The overall system is simply viewed as a number of service marketplaces.